Secretion ofAspergillus oryzaeAlkaline Protease in an Osmophilic Yeast,Zygosaccharomyces rouxii

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Secretion of Aspergillus oryzae alkaline protease in an osmophilic yeast, Zygosaccharomyces rouxii.

To produce Aspergillus oryzae alkaline protease (Alp) in an osmophilic yeast Zygosaccharomyces rouxii, we constructed an expression plasmid consisting of the Z. rouxii glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter, the prepro-Alp cDNA of A. oryzae, the whole sequence of Z. rouxii plasmid pSR1, and the G418 resistant gene. The resulting plasmid, when introduced into Z. rouxii cells, ...

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Heterothallism in Saccharomyces rouxii.

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1990

ISSN: 0002-1369

DOI: 10.1080/00021369.1990.10870348